Journal: PLoS ONE

Article Title: Subtype-specific characterization of breast cancer invasion using a microfluidic tumor platform

doi: 10.1371/journal.pone.0234012

Figure Lengend Snippet: (A) Microanatomical replication of IDC-on-chip. Epithelial cancer cells at the lining of the lumen structure formed through collagen gel. (B) 3D reconstruction of MCF-7 lumen structure in IDC-on-chip platform and cross-sectional images. Magenta stains are plasma membrane and blue are nuclei. (C) E-cadherin (E-cad, green) immunostaining of MCF-7 duct with nuclei (blue). Scale bars indicate 100μm.

Article Snippet: For the next step, the primary antibodies, E-cadherin (Abcam, MA) were added to M.O.M. diluent (Vector Laboratory BMK2202) and 4 drops of Biotin solution per 1 mL of diluent at a 1:100 dilution.

Techniques: Immunostaining

Journal: PLoS ONE

Article Title: Subtype-specific characterization of breast cancer invasion using a microfluidic tumor platform

doi: 10.1371/journal.pone.0234012

Figure Lengend Snippet: (A) Structure and cell morphology at the duct in IDC-on-chip. Histological staining of the cells in the duct was performed with Haematoxylin and Eosin (H&E) at day 3 of culture. (B) Evaluation of cell proliferation using BrdU assay. (C) Expression of N-cadherin at the protrusions. Nuclei (blue) and N-cadherin (red). N-cadherin is a cell-cell adhesion marker important for migratory and invasive behavior of tumoral cells.

Article Snippet: For the next step, the primary antibodies, E-cadherin (Abcam, MA) were added to M.O.M. diluent (Vector Laboratory BMK2202) and 4 drops of Biotin solution per 1 mL of diluent at a 1:100 dilution.

Techniques: Staining, BrdU Staining, Expressing, Marker

Journal: Frontiers in Toxicology

Article Title: Characterization of a human thyroid microtissue model for testing thyroid disrupting chemicals

doi: 10.3389/ftox.2024.1408808

Figure Lengend Snippet: Characterization of the cell population from five donor lots determined by FLOW. (A) Percentage of cells stained positive for EpCAM, CD90, and CD144. > 0.5 × 10 6 viable cells were used for each of the five donor lots. (B) Representative images of thyrocyte cells from donor lots 2217737 (top row) and 2214495 (bottom row) in 2D culture stained for Cytokeratin 7 (CK7, green), Fibroblast-specific protein 1 (FSP1, red), and DAPI (blue), and three channels merged. Magnification ×50. Scale bar = 200 µm. (C) Representative images of microtissues in 3D culture seeded at 7,500 cells per well and (D) levels of Thyroxine (T 4 ) from microtissues in 3D culture on day 14. Cells were treated with Thyroid Stimulating Hormone (1.0 mIU/mL). Magnification ×40. Scale bar = 50 µm. Error bars represent standard deviation. n ≥ 3 replicates from five donor lots.

Article Snippet: Anti-human Epithelial Cell Adhesion Molecule (EpCAM) (CD326, clone: VU-1D9)- FITC (Stem Cell Technologies, Vancouver, BC), anti-human CD90 (Thy-1, clone: 5E10)- Alexa Fluor 700 (ThermoFisher Scientific), and anti-human CD144 (clone: 55-7H1)- PerCPCy5.5 (BD Biosciences) were used for thyrocyte staining at 1:100 dilution.

Techniques: Staining, Standard Deviation